Hello, welcome to CRISPR-TAPE, a protein-centric gRNA design tool.

Follow this link, download the relevant executable application for your operating system, and then have a play!

And head here for an accompanying manuscript describing the tool in more detail.

The Toxoplasma gondii GT1 genome file is preloaded in the application, and so no need to do anything if that’s your organism of interest.

Click here for the README to get full details of how to use the tool. Alternatively, following installation, simply launch the program and then hit the “instructions” button.

Have fun!